Protein Expression Screening Highly Customized Service

Save time and resources by testing various expression hosts, constructs and expression parameters in parallel to find the best system for expression of your target protein. Our customized protein expression screening service offers simultaneous screening of your target protein expression in different expression hosts such as E. coli, yeast, insect cells and mammalian cells, by using different expression vectors under various expression conditions (> 60 different vectors available).

Expression Host Screening

Recombinant expression of a new, unknown or very difficult target protein is often performed sequentially in different hosts and needs a deep expertise on various expression systems. This takes a lot of valuable time and makes costs unpredictable. Our host protein expression screening service provides a cross organism expression platform which enables parallel screening of protein expression in different expression organisms and strains to detect the best system for your individual needs.

  • Parallel protein expression screening in different organisms, strains and constructs saves time and money
  • Various expression organisms available: E.coli, yeast, insect and mammalian cells
  • Longstanding experience on different expression systems and on expression vector engineering
  • Expression vector features tailored to your individual needs
  • Clear and comprehensive documentation
  • Free project consultation with our scientific experts

Additional Service Options:

  • Upscaling of protein expression
  • Complete up- and downstream process development
  • Protein expression and purification optimization
  • Protein activity screening
  • Extended analytics, such as mass spectrometry analysis and solubility analysis

 

Fig.1: Sequential vs. parallel model – in comparison to the conventional host finding strategy the optimized expression host screening saves maximum of time and is highly efficient.

 

Protein Productivity Screening – Selection of the best E.coli expression system/conditions

E.coli is often chosen as the preferred expression host, as it is usually a robust and cost-effective system. However, many protein targets are expressed poorly in E. coli or are synthesized as insoluble, inactive inclusion bodies. Our protein productivity screening service offers high-throughput screening of combinations of different expression vectors, expression strains and appropriate media using a 48-well based microfermenter system (BioLector) which monitors growth, disolved oxygen (DO), pH and fluorescence in every well. This protein productivity screen allows us to identify the best expression construct, strain and conditions to express and upscale production of your target.

  • High-throughput protein expression screening – the fastest way to identify the best expression strain and condition
  • Parallel screening of several ready-to-go expression constructs, strains and conditions saves time and money
  • Expression conditions and constructs are optimized for protein yield and activity. For example: expression strain, expression conditions (media, temperature, induction conditions), choice of expression vector/promotor, type and position of affinity tag,  promotor regions (strong/weak expression), fusion tags with special features
  • Comprehensive documentation
  • Free project consultation with our scientific experts

Additional Service Options:

  • Upscaling of protein expression
  • Complete up- and downstream process development
  • Protein expression and purification optimization
  • Protein activity screening
  • Extended analytics, such as mass spectrometry analysis and solubility analysis

 

Fig.2: One possible set-up to test up to 48 conditions in parallel.

Reinhold Horlacher, PhD

Reinhold Horlacher, PhD

Managing Director & CSO

We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.

Get In Contact With Our Scientific Experts

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