Recombinant Protein Expression in Mammalian Cells – Guaranteed Service Package
Guaranteed expression and purification service packages for recombinant protein expression in mammalian cells are available for target sequences that are determined as standard proteins by our feasibility check during initial free individual consulting with our scientific experts. Our packages include all steps from cloning to purification for a fixed price. We guarantee the final yield, purity and timeline – you only pay when we succeed!
Process of Recombinant Protein Expression in Mammalian Cells
Guaranteed Protein Expression Package Includes
- All-inclusive from cloning to purification for a fixed price!
- Free feasibility check and dedicated consulting
- Guaranteed yield: starting at 1 mg purified protein
- Guaranteed purity: >75 % protein purity
- Guaranteed processing time: 2 business weeks
- Comprehensive datasheet
- Standard analytics: SDS Page, purity check, concentration determination
- Guaranteed service – pay only when we succeed!
Additional service options:
- Gene synthesis as starting point
- Purchase of additionally produced protein
- Pilot study for demanding targets to determine productivity
- Extended analytics, such as mass spectrometry, endotoxin detection, western blot or ELISA
- Low endotoxin purification and endotoxin removal
“We highly valued the fast and excellent communication and the high flexibility of the team! For any future project we will preferably entrust in Trenzyme’s protein production services.”
Your benefits from working with us
Guarantee – Pay Only When We Succeed!
We guarantee the final quantity and purity of your desired target protein in the given timeframe. You only pay when we succeed!
All-inclusive Service Package
We provide a high quality service package for recombinant protein expression in mammalian cells. Everything from cloning to final protein purification is included in a fixed price.
Transparency & High Quality
Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.
Free Feasibility Analysis & Consulting
Our free feasibility check of your protein sequence is the basis for our free individual consultation with you to define the ideal service strategy for your target that will lead to success.
Free Feasibility Check
During initial free individual consulting, our scientific experts run a feasibility check (e.g. literature check, database check, product check, etc.) with your protein sequence to define the ideal service strategy for your specific target:
- If your target sequence is determined by our initial check tools as feasible protein, we offer the guaranteed service package.
- If your target sequence is determined as demanding protein or you requested a high yield production (> 10 mg), we offer highly modular custom-tailored solutions.
Mammalian Expression FAQs
My target protein should be glycosylated - which expression host do you recommend?
For generation of glycosylated proteins we offer different expression systems like Yeasts, insect cells (Baculo) or also mammalian cell lines. Depending on the nature of your target protein and your exact needs, we will propose the most suitable expression host and system for your project.
What starting materials are required to begin a project?
We only need the amino acid sequence or uniprot reference number of your desired protein.
Why does my protein show a higher (or lower) MW on SDS-PAGE or Western blot as calculated?
Posttranslational modifications like glycosylation or very rigid protein structures may have influences on protein properties and running behaviour. Sometimes proteins run higher, seldom lower and sometimes they run as a smear.
Reinhold Horlacher, PhD
Managing Director & CSO
We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.
Get In Contact With Our Scientific Experts