Labeled Protein Expression in E.coli – Guaranteed Service Package
We provide a high quality guaranteed service package including the generation of an expression plasmid, recombinant 13C, 15N or D2O labeled protein expression in E. coli and target protein purification for a fixed price. For target sequences that are determined as suitable proteins by our feasibility check during initial free individual consulting with our scientific experts, we guarantee the final yield, purity and timeline – you only pay when we succeed!
Process of Labeled Protein Expression in E. coli
Guaranteed Protein Expression Package Includes
- All-inclusive from cloning to purification for a fixed price!
- Free feasibility check (see below) and dedicated consulting
- 15N,13C or D2O protein labeling included
- Guaranteed yield: starting at 1 mg purified protein
- Guaranteed purity: >75% protein purity
- Guaranteed processing time: 2 business weeks
- Comprehensive datasheet
- Standard analytics: SDS Page, purity check, concentration determination
- Guaranteed service – pay only when we succeed!
Additional service options:
- Gene synthesis as starting point
- Purchase of additionally produced protein
- Pilot study for demanding targets to determine productivity
- Production of large amounts of labeled proteins by high cell density fermentation
- Extended analytics, such as mass spectrometry, endotoxin detection, western blot or ELISA
- Low endotoxin purification and endotoxin removal
“It was a pleasure to work with trenzyme. Our expectations were fully met. trenzyme’s team of professional scientific experts helped us to complete our objectives in time. We were also pleased with the quality of the documentation provided. In a nutshell, I can recommend trenzyme as a reliable service provider.”
Guarantee – Pay Only When We Succeed!
We guarantee the final quantity and purity of your desired target protein in the given timeframe. You only pay when we succeed!
All-inclusive Service Package
We provide a high quality service package for recombinant 13C, 15N or D2O labeled protein expression in E. coli. Everything from cloning to final protein purification and protein labeling is included at a fixed price.
Transparency & High Quality
Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.
Free Feasibility Analysis & Consulting
Our free feasibility check of your protein sequence is the basis for our free individual consultation with you to define the ideal service strategy for your target that will lead to success.
Free Feasibility Check
During initial free individual consulting, our scientific experts run a feasibility check (e.g. literature check, database check, product check, etc.) with your protein sequence to define the ideal service strategy for your specific target:
- If your target sequence is determined by our initial check tools as feasible protein, we offer the guaranteed service package.
- If your target sequence is determined as challenging protein or you requested a high yield production (> 10 mg), we offer highly modular custom-tailored solutions.
Labeled Proteins FAQs
Is labeled high-yield protein production also available?
Yes, we have developed a protocol for high-yield production of labeled proteins in bioreactors at high-cell densities.
Is double or triple labeling for guaranteed packages possible?
Double and triple labeling are possible. We offer the following labels, which can be combined in different combinations: 15N, 13C, D2O.
Reinhold Horlacher, PhD
Managing Director & CSO
We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.
Get In Contact With Our Scientific Experts