Recombinant Protein Expression and PurificationHighly Customized Services

Our experienced scientific experts provide comprehensive and customized recombinant protein expression and purification services according to your specific project requirements. As your reliable research partner, we are dedicated to providing tailored solutions for your individual research project. Due to the extensive knowledge of our experts, the use of different hosts (E.coli, baculovirus, yeast, mammalian cells) for the expression of your gene of interest is no problem.

 

Highly Customized Solution for Your Target Expression

We provide free individual consulting and modular systems to develop a highly customized and flexible project strategy that is tailored to your needs. We work in close collaboration with our clients to ensure high quality and efficiency. Our dedicated project management will guide your project through every step of the process and keep you constantly informed about the project progress via continued status reports. Due to the constantly exchange of information between you and our scientific experts, we react with highest flexibility to potential project strategy changes.

 

Our Recombinant Protein Expression and Purification Services

Recombinant Protein Expression Services

  • Extensive knowledge on different expression systems: E. coli, baculovirus, yeast and mammalian cells
  • Collection of diverse ready-to-go expression vectors (vector toolbox)
  • Process development and upscaling according to your individual project requirements
  • Expression host screening and productivity screening
  • Optimization of recombinant protein expression
  • Optimization of soluble protein production
  • High expression yields [mg-g] for production in bacteria (E. coli), yeast and mammalian cells possible

 

Recombinant Protein Purification Services

  • State-of-the-art purification strategies
  • Optimization of purification process
  • Refolding of denatured proteins
  • Endotoxin free purification or endotoxin removal
  • Protein characterization
  • Extended quality control according to your needs
  • Implementation of activity assay possible

 

General Services

  • Highly individualized and flexible strategies for your recombinant protein expression and purification
  • Extensive services for challenging proteins of interest
  • Process development including comprehensive report
  • Detailed documentation of all project steps by a Laboratory Information Management System (LIMS)
  • High quality standards (DIN EN ISO 9001:2015 certified company)
  • Continued feedback about project process leads to maximum project success
  • Extended optional feasibility analysis according to your specific research project requirements
  • DNA cloning into your desired vector or into our proprietary expression vector saves time and money
  • Gene synthesis with codon optimization as starting point if desired

 

„Due to the outstanding flexibility to address our particular project needs as well as the excellent quality of the provided services, trenzyme is one of our preferred service providers since many years.”

Dr. Steffen Schaffer

Evonik Creavis GmbH, Marl, Germany

 

Custom Protein Production Service - Overview

 

Custom Production FAQs

What kind of analysis of the produced proteins do you offer?

We offer different analytical methods according to your needs. Always included is SDS-PAGE and determination of protein concentration. Very often we do Western blot analysis. If needed, we can determine the oligomeric state by analytical size exclusion chromatography (SEC) or native PAGE. Mass spectroscopic analysis is also offered on request to determine for example the modification of proteins.

Does trenzyme offer recombinant proteins off the shelf?

We are currently working on off the shelf proteins. Simply get in contact with us and maybe we have your protein already available.

How many custom proteins have you produced so far? What is the typical success rate?

We are working on recombinant proteins since almost 20 years and we have successfully produced more than 1000 different proteins so far. The generall success rate is over 80%.

 
Reinhold Horlacher, PhD

Reinhold Horlacher, PhD

Managing Director & CSO

We would be happy to provide you with high quality support on your protein research project. Use our contact form to let us know your questions and requests concerning our highly customized services for recombinant protein expression and purification. Our scientific experts will reply shortly.

Get In Contact With Our Scientific Experts for Custom Protein Production







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