SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), lyophilized formulation

Recombinant protein of the receptor binding domain (RBD) of SARS-CoV-2 (COVID-19) Spike S1 from Wuhan pneumonia virus with C-terminal His-Tag, lyophilized formulation.

Order Details

  • 100µg – 300€
  • more on request – bulk and gram quantities available

Shipped next working day

Overview

Product NameSARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), lyophilized formulation
Catalog No.P2020-021
RefSeq LinksNC_045512.2; MN908947.3; YP_009724390.1; QHD43416.1; GeneID: 43740568
SynonymsSARS-CoV-2; coronavirus; SARS-CoV-2 spike RBD; SARS-CoV-2 spike protein; 2019-nCoV; COVID-2019; COVID-19

Sequence Information

SpeciesSARS-CoV-2; Wuhan seafood market pneumonia virus
TagsHis-tag, C-terminal
Sequence without tags (AA 319-541)RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYA
DSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAG
STPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Product Information

Expression Hosthuman, HEK293
FormulationPBS, pH 7,4
FormatLyophilized and shipped at room temperature
Purity> 85% as determined by SDS-PAGE

Background Information

The spike (S) glycoprotein of coronaviruses is essential for binding of the virus to the host cell at the beginning of the infection process. The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein is responsible for membrane fusion and is therefore required for virus entry and cell fusion. The target protein is also a major immunogen and a possible target for entry inhibitors.
The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (RBD) responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2). The S2 subunit mediates fusion between the viral and host cell membranes. The S1 RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

SARS-CoV-2 Spike S1 (RBD) recognizes hACE2 (ECD) with an affinity constant of 1 nM as verified by biolayer interferometry

SARS-CoV-2 Spike S1 (RBD) is highly pure and forms stable monomers of 28 kDa as verified by SEC

Biolayer interferometry binding analysis graph

Determination of elution profile by absorbance at 289 nm (blue line).

Biolayer interferometry binding analysis (green lines) of hACE2 (ECD, processed), tag-free to immobilized SARS-CoV-2 Spike S1 (RBD), His-tag on Ni-NTA Dip and Read™ Biosensors. Grey lines correspond to a global fit of the data using a 1:1 binding model.
Device: Octet RED96e, ForteBio.

Size exclusion chromatography (SEC) of purified SARS-CoV-2 Spike S1 (RBD), His-tag protein, determination of elution profile by absorbance at 280 nm (blue line).

 

Fluorescence chromatogram

Analysis of released N-glycans by HILIC (example data) of SARS-CoV-2 Spike S1 (RBD)

Analysis of released N-glycans by HILIC (example data). More and lot specific analytical data available on request (provided by our partner Biofidus AG).

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

SDS-PAGE/Coll.Coomassie of SARS-CoV-2 Spike S1Histogram (of marked lane in gel picture) of SARS-CoV-2 Spike S1 Protein

 

Further recombinant SARS-CoV-2 proteins

Learn more about our other recombinant SARS-CoV-2 proteins.
Reinhold Horlacher, PhD

Reinhold Horlacher, PhD

Managing Director & CSO

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