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SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), lyophilized formulation
Recombinant protein of the receptor binding domain (RBD) of SARS-CoV-2 (COVID-19) Spike S1 from Wuhan pneumonia virus with C-terminal His-Tag, lyophilized formulation.
|Product Name||SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), lyophilized formulation|
|RefSeq Links||NC_045512.2; MN908947.3; YP_009724390.1; QHD43416.1; GeneID: 43740568|
|Synonyms||SARS-CoV-2; coronavirus; SARS-CoV-2 spike RBD; SARS-CoV-2 spike protein; 2019-nCoV; COVID-2019; COVID-19|
|Species||SARS-CoV-2; Wuhan seafood market pneumonia virus|
|Sequence without tags (AA 319-541)||RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYA|
|Expression Host||human, HEK293|
|Formulation||PBS, pH 7,4|
|Format||Lyophilized and shipped at room temperature|
|Purity||> 85% as determined by SDS-PAGE|
|The spike (S) glycoprotein of coronaviruses is essential for binding of the virus to the host cell at the beginning of the infection process. The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein is responsible for membrane fusion and is therefore required for virus entry and cell fusion. The target protein is also a major immunogen and a possible target for entry inhibitors.|
The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (RBD) responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2). The S2 subunit mediates fusion between the viral and host cell membranes. The S1 RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
SARS-CoV-2 Spike S1 (RBD) recognizes hACE2 (ECD) with an affinity constant of 1 nM as verified by biolayer interferometry
SARS-CoV-2 Spike S1 (RBD) is highly pure and forms stable monomers of 28 kDa as verified by SEC
Biolayer interferometry binding analysis (green lines) of hACE2 (ECD, processed), tag-free to immobilized SARS-CoV-2 Spike S1 (RBD), His-tag on Ni-NTA Dip and Read™ Biosensors. Grey lines correspond to a global fit of the data using a 1:1 binding model.
Size exclusion chromatography (SEC) of purified SARS-CoV-2 Spike S1 (RBD), His-tag protein, determination of elution profile by absorbance at 280 nm (blue line).
Further recombinant SARS-CoV-2 proteins
Reinhold Horlacher, PhD
Managing Director & CSO
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