Have you ever postponed transfection experiments because you forgot to thaw your CHO, HEK, or HeLa cells? Did you stay in the lab from sunrise to sunset because your freshly thawed cells have to adapt to the culture at least a few hours before they can be efficiently transfected? With FreezeFect CHO, HEK and HeLa cells this is history.

FreezeFect cells are ready-to-use transfection competent cells right off the cryogenic storage. Transfect mammalian cells with the same degree of convenience you are used from E. coli.

FreezeFect cells are generated based on a proprietary Trenzyme technology, which was licensed to Lonza in 2011. If you need to generate your own transfection competent cells, the technology is available as Nucleofector™ PLUS supplements, which can be used in conjunction with all existing Nucleofector™ kits.

No matter which kit and Nucleofector™ device you are working with, just substitute the standard supplement (delivered with regular Nucleofector™ kit) with the appropriate Nucleofector™ PLUS supplement.  

• Cryopreserved transfection competent cells ready-to-use any time
• Elimination of variations caused by the cell culture process
• Each FreezeFect batch is quality certified and will show excellent transfection efficiency and reproducibility over a period of more than one year
• Works with any Nucleofector™ device

Trenzyme recommends Lonza Nucleofection™PLUS technology!