EndoOUT

With EndoOUT, we provide our customers a knockdown system that allows efficient selection of mammalian cells with endogenous depletion of a protein of interest. This system allows for rapid and reliable generation of homogenous populations of cells, readily usable as assay cell line.

By using a bicistronic vector, the knockdown cassette is expressed together with an antibiotic resistance on a single RNA, which directly links knockdown efficiency with applied selection pressure.

This property renders the screening of large numbers of colonies to identify clones that can be used for functional analyses unnecessary and reduce the time consumption for the generation of stable clones by at least 50 % compared to other available knockdown systems.

EndoOUT can be combined with ExoIN.

The EndoOUT system as a tool for rapid selection of a homogenous knockdown population of cells.

The primary transcript is finally processed to a functional siRNA and a translatable Puromycin resistance gene. Co expressed with a Tetracycline repressor, the transcription can be induced by addition of Tetracycline or its analog Doxycyclin.

Further information